ELISA
First mobile version of the Enzyme Linked Immunosorbent Assay (ELISA), the gold standard in biochemical analysis. Courtesy of Arsenii Zhdanov

Patients to Skip the Lab, Get Immediate Results With New Blood Test Technology

The often costly, time-consuming process of obtaining bloodwork and urinalysis could soon get a major overhaul. Researchers at the University of South Florida in Tampa have created a mobile version of the "Enzyme Linked Immunosorbent Assay" (ELISA), the gold standard technique used to detect the presence of an antibody or antigen. Instead of sending patients to a laboratory, new cellphone-based technology allows for the very same test to be conducted in the doctor's office, clinic or even in a remote area.

"ELISA is an important technology for biochemical analysis of proteins and hormones and is critical for the diagnosis of many diseases, such as HIV and Lyme disease," said corresponding author Anna Pyayt, PhD, assistant professor at the University of South Florida College of Engineering. "But the machines required for the incubation and reading are expensive and bulky. The MELISA allows patients to undergo testing and obtain results at point-of-care."

The Mobile Enzyme Linked Immunosorbent Assay (MELISA) is detailed in an article just published in Biosensors and Bioelectronics. The device accurately measures progesterone levels, a key hormone that impacts female fertility and is indicative of some cancers. MELISA consists of a water bath heater that incubates samples at a target temperature and analyzes them via images taken by mobile phone. It uses color analysis to determine the RGB (red, green, blue) color components of each sample. The blue color component is used for further analysis due to its sensitivity to the changes in progesterone concentration.

"It is designed to make biomedical testing simple and affordable. When low cost testing can be integrated with routine clinic visits, this would greatly improve the quality of healthcare and detect worrisome signs earlier, Pyayt said.

The portable MELISA weighs just one pound, dramatically helping older patients suffering chronic conditions and those across the world.  Pyayt and her team at USF are now calibrating MELISA for a variety of tests including testosterone. With those results, they'll apply for FDA approval so clinics can start using the device.

Source: University of South Florida

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