For the first time,
"This is the first time that anyone has identified the nutrients that sustain Salmonella while it is infecting a host's body," says Dr. Arthur Thompson from the
The nutrition of bacteria during infection is an emerging science. This is one of the first major breakthroughs, achieved in collaboration with Dr. Gary Rowley at the
Salmonella food poisoning causes infection in around 20 million people worldwide each year and is responsible for about 200,000 human deaths. It also infects farm animals and attaches to salad vegetables.
During infection, Salmonella bacteria are engulfed by immune cells designed to kill them. But instead the bacteria multiply.
Salmonella must acquire nutrients to replicate. The scientists focused on glycolysis, the process by which sugars are broken down to create chemical energy. They constructed Salmonella mutants unable to transport glucose into the immune cells they occupy and unable to use glucose as food. These mutant strains lost their ability to replicate within immune cells, rendering them harmless
"Our experiments showed that glucose is the major sugar used by Salmonella during infection," said Thompson.
The mutant strains still stimulate the immune system, and the scientists have filed patents on them which could be used to develop vaccines to protect people and animals against poisoning by fully virulent Salmonella.
Glycolysis occurs in most organisms including other bacteria that occupy host cells. Disrupting how the bacteria metabolise glucose could therefore be used to create vaccine strains for other pathogenic bacteria, including superbugs.
The harmless strains could also be used as vaccine vectors. For example, the flu gene could be expressed within the harmless Salmonella strain and safely delivered to the immune system.
The next stage of the research will be to test whether the mutants elicit a protective immune response in mice.