Discovery Offers Insight Into Treating Norovirus

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Shown here is the electron microscopy image reconstruction of mouse norovirus (MNV). The capsid is colored according to the distance from the center of the virion. The inner shell is colored red and yellow while the protruding domain (P domain) is colored in green and blue. The outer most tip (blue) is used by the virus to recognize the host cell and is where many antibodies bind. There is a yellow strand connecting the P domains to the shell of the virus that likely helps keep the P domains highly mobile and has been now observed in three different genera of this family of viruses.

Twenty million Americans get sick from norovirus each year according to data released by the Centers for Disease Control (CDC). Often called vomiting illness, it can spread rapidly on cruise ships, and in dormitories and hospitals.  Recent data from the CDC shows deaths from gastrointestinal infections have more than doubled and have become a particular threat to the elderly.  The virus is shed in the stool of the infected individual, has a short incubation period and can spread quickly if proper hand washing and other measures are neglected.
 
While researchers say that vaccines for intestinal infections are among the most difficult to develop, a recent discovery may provide the critical information needed for success.

“Sometimes atomic structure gives us clues on how viruses work and how to make better vaccines,” says Dr. Thomas Smith, principal investigator at the Donald Danforth Plant Science Center, whose recent article, "Structural Basis for Broad Detection of Genogroup II Noroviruses by a Monoclonal Antibody That Binds to a Site Occluded in the Viral Particle" was included in the Journal of Virology. 

Smith was part of a team of scientists lead by Dr. Peter D. Kwong of the National Institute of Allergy and Infectious Diseases. Their research demonstrated that the virus has a structure unlike that of other viruses in that is has protein “lollipop” like structures that likely gives it more flexibility in attaching to cells.  There are four genera of this virus family, the Caliciviruses, with the Sapoviruses and Noroviruses being the major cause of severe gastroenteritis in humans.
 
Smith and his colleagues discovered that because of the “lollipop” structure, antibodies against the norovirus may be able to bind to the more conserved underside of this floppy structure.  This suggests that the extreme flexibility of the norovirus particle may allow for antibody recognition of protected surfaces that might otherwise be buried on intact particles.
 
This information will give researchers more insight on how to manipulate complex viruses as well as to design and develop better drugs to treat the maladies they cause.  Rotovirus, a member of a different viral family but also causes severe gastro intestinal distress primarily in children, is being well controlled by the recent development of a vaccine.
 

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