Building upon previous efforts producing a high-quality de novo genome assemblies of deadly 2011 E.coli O104:H4 outbreak strain, the BGI and their collaborators at the University Medical Centre Hamburg-Eppendorf have now released the first complete map of the genome and plasmids without any assembly gaps.
This final draft of the genome shows the disease strain has a circular chromosome 5,278 kbp in length, and three additional plasmids 88 kbp, 75 kbpand 1.5 kbp in size, respectively. The chromosome contains around 5,000 predicted coding sequences (CDSs), covering 87.09 percent of the genome. The biggest plasmid is highly homologous to a previously sequenced plasmid isolated from a horse and carryingadditional multi-drug resistance genes; the smallest one is a so-called "selfish plasmid" carrying only two genes, one of which encodes a DNA replication protein; the other carrying the aggregative adherence fimbria I (AAF/I) gene cluster, which is associated with E. coli aggregation ability and virulence, and likely to play a role in the persistence of the disease.
BGI researchers found that the Shiga-toxin-encoding genes, responsible for most of the pathogenicity of the disease, were likely encoded by a viral prophage that integrated in the bacterial chromosome. Several insertion hotspots, including one nested multi-antibiotic resistant associated locus, were also identified in the research. This indicates that horizontal gene transfer events may have played important roles in the evolution of virulence and drug resistance of this strain.
The BGI says results of these and previous phylogenetic and comparative genomic analyses now gives researchers the confidence to confirm that the outbreak strain belong to an EAEC (enteroaggregative E. coli)lineage, but acquired the Shiga toxin producing ability by the integration of a phage genome. Researchers add that this explains the initial confusion as to why the EAEC-lineage bacteria harbored some characteristicsof EHEC (enterohaemorrhagic E. coli) strains. Therefore, the deadly Germany E. coli is not a completely new bacteria and can be considered a "hybrid" strain, now temporarily termed Shiga toxin-producing enteroaggregative Escherichia coli (STpEAEC).