Researchers Explore Effect of Low-Dose Gaseous Ozone on Pathogenic Bacteria

Article

Treatment of chronically infected wounds is a challenge, and bacterial environmental contamination is a growing issue in infection control. Ozone may have a role in these situations. Fontes, et al. (2012) sought to determine whether a low dose of gaseous ozone/oxygen mixture eliminates pathogenic bacteria cultivated in Petri dishes.
 
A pilot study with six bacterial strains was conducted using different concentrations of ozone in an ozone-oxygen mixture to determine a minimally effective dose that completely eliminated bacterial growth. The small and apparently bactericidal gaseous dose of 20 mug/mL ozone/oxygen (1:99) mixture, applied for 5min under atmospheric pressure was selected. In the 2nd phase, eight bacterial strains with well characterized resistance patterns were evaluated in vitro using agar-blood in adapted Petri dishes (105 bacteria/dish). The cultures were divided into three groups: 1) ozone-oxygen gaseous mixture containing 20 mug of O3/mL for 5 minutes; 2) 100 percent oxygen for 5 minutes; 3) baseline, no gas was used.
 
The selected ozone dose was applied to the following eight strains: Escherichia coli, oxacillin-resistant Staphylococcus aureus, oxacillin-susceptible Staphylococcus aureus, vancomycin-resistant Enterococcus faecalis, extended-spectrum beta-lactamase-producing Klebsiella pneumoniae, carbapenem-resistant Acinetobacter baumannii, Acinetobacter baumannii susceptible only to carbapenems, and Pseudomonas aeruginosa susceptible to imipenem and meropenem. All isolates were completely inhibited by the ozone-oxygen mixture while growth occurred in the other two groups.
  
The researchers concluded that a single topical application by nebulization of a low ozone dose completely inhibited the growth of all potentially pathogenic bacterial strains with known resistance to antimicrobial agents. Their research was published in BMC Infectious Diseases. 

Reference: Fontes B, et al. Effect of low-dose gaseous ozone on pathogenic bacteria. BMC Infectious Diseases 2012, 12:358 doi:10.1186/1471-2334-12-358

Newsletter

Stay prepared and protected with Infection Control Today's newsletter, delivering essential updates, best practices, and expert insights for infection preventionists.

Recent Videos
Dirty white towels on the floor used to clean up orange or red liquid. (Image credit AI by Adobe Stock)
Infection Control Today Educator of the YearTM Award for 2025
 Futuristic UV Sanitizer with Sleek Design on a white background.  (Adobe Stock 1375983522 by Napa)
© 2025 MJH Life Sciences

All rights reserved.