Virucidal Activity of Airborne Disinfectants is Tested on Dried Poliovirus

The activity of airborne disinfectants on bacteria, fungi and spores has been reported. However, the issue of the virucidal effect of disinfectants spread by fogging has not been studied thoroughly. Thevenin, et al. (2013) developed a procedure to determine the virucidal activity of peracetic acid-based airborne disinfectants on a resistant non-enveloped virus poliovirus type 1.

This virus was laid on a stainless carrier, and the products were spread into the room by hot fogging at 55 degrees C for 30 minutes at a concentration of 7.5mL.m-3. Poliovirus inoculum, supplemented with 5 percent heat inactivated nonfat dry organic milk, were applied into the middle of the stainless steel disc and were dried under the air flow of a class II biological safety cabinet at room temperature. The viral preparations were recovered by using flocked swabs and were titered on Vero cells using the classical Spearman-Karber CPE reading method, the results were expressed as TCID50.ml-1.

The infectious titer of dried poliovirus inocula was kept at 105 TCID50.mL-1 up to 150 minutes at room temperature. Dried inocula exposed to airborne peracetic acid containing disinfectants were recovered at 60 and 120 minutes post-exposition and suspended in culture medium again. The cytotoxicity of disinfectant containing medium was eliminated through gel filtration columns. A 4-log reduction of infectious titer of dried poliovirus inocula exposed to peracetic-based airborne disinfectant was obtained.
 
The researchers say this study demonstrates that the virucidal activity of airborne disinfectants can be tested on dried poliovirus. Their research was published in BMC Infectious Diseases.

Reference: Thevenin T, Lobert PE and Hober D. Inactivation of an enterovirus by airborne disinfectants. BMC Infectious Diseases 2013, 13:177 doi:10.1186/1471-2334-13-177