Drug-Resistance Fears for Deadly Fungal Disease

Deadly human fungal infections caused by certain strains of Aspergillus fungi appear to be developing resistance to current drug treatments at an alarming rate, say scientists.

University of Manchester researchers, working with colleagues in Newark, N.J., have developed a new test that can not only better diagnose Aspergillus infection, but can also spot signs of antifungal resistance to azoles the class of drugs used to treat patients with aspergillosis.

Using the new test, which uses direct molecular detection rather than culturing the fungus in a Petri dish, the team found that 55 percent of aspergillosis patients had telltale signs known as 'markers' that indicated they had developed resistance to azoles. The findings compare to resistance rates of 28 percent carried out by the team just two years ago using traditional culturing methods.

Furthermore, the study published in the journal Clinical Infectious Diseases discovered azole-resistance markers in three-quarters of the small number of aspergillosis patients (eight) whom had never been treated with an azole, suggesting widespread dissemination of resistance.

"Aspergillus significantly worsens asthma symptoms and causes life-threatening infections in those with long-term lung infections or damaged immune systems, such as chemotherapy and transplant patients or people with HIV," says David Denning, professor of medicine and medical mycology at the University of Manchester and director of the National Aspergillosis Centre at the University Hospital of South Manchester NHS Foundation Trust.

"Using an ultrasensitive, real-time test for Aspergillus, similar to the method used to diagnose HIV, MRSA and influenza, we have directly detected azole resistance in people with aspergillosis, without first culturing the fungus in a dish," Denning says. "The presence of Aspergillus was detected in many more samples than using traditional culture methods, and 55 percent were found to contain azole-resistance markers. This is an extraordinarily high rate of resistance, possibly related to fungicide use in agriculture more than a third of 'pesticides' used by UK farmers are azoles and long treatment courses in patients, so the findings have major implications for the sustainability of azoles for human antifungal therapy."

The azoles itraconazole (Johnson & Johnson), voriconazole (Pfizer) and posaconazole (Merck) have annual sales of more than $1 billion annually. Conventional diagnosis of aspergillosis is limited by poor culture yield, and so the true frequency of azole resistance has been unclear.

In this latest study, the researchers analyzed phlegm from patients with allergic and chronic lung disease caused by Aspergillus and found that almost twice the proportion of individuals tested had resistance markers in their sample compared to a Petri dish (or culture) study carried out by the team in 2008-2009.

Denning adds, "Not only is molecular testing much more sensitive than conventional culture for diagnosis, but it enables testing for resistance, which until now has been impossible if cultures are negative. Given the rising frequency of resistance in Aspergillus in northern Europe, China and the United States, this study provides key data for doctors to shift antifungal therapy in the face of resistance."