The parasite responsible for a form of malaria now spreading from macaques to humans in South Asia could evolve to infect humans more efficiently, a step toward enhanced transmission between humans, according to a new study from Harvard T.H. Chan School of Public Health. The researchers say that defining the means by which the Plasmodium knowlesi parasite invades red blood cells could lead to interventions to prevent the emergence of the zoonosis into the human population.
The researchers identified a sugar variant on the surface of human red blood cells that currently limits the ability of P. knowlesi to invade, and demonstrated that the monkey malaria parasite has the ability to evolve to get around this barrier and pass into the human population in a more virulent form.
The study will appear online April 4, 2016 in Nature Communications.
"With increasing concern about the spread of P. knowlesi into human populations, it is great to be able to gain insight into what the molecular stumbling blocks are for P. knowlesi infection of humans, and how the parasite can potentially overcome them," said first author Selasi Dankwa, who carried out the work while a doctoral student in the Department of Immunology and Infectious Diseases at Harvard Chan School.
The macaque malaria parasite P. knowlesi has emerged as a major source of human infections in Southeast Asia, as the monkey's habitats are encroached upon through logging and farming. While most human infections are mild, increasing numbers of severe infections are being reported, leading to concerns that the parasite is adapting to infect humans more efficiently.
The researchers used a unique stem cell-based genetic approach for interrogating the host red blood cell to explore the parasite's ability to invade and adapt. They did an experiment that introduced the macaque sugar variant onto the human red blood cell surface and demonstrated that the parasite normally dependent on the macaque variant for invasion was unable to use the human version, limiting its virulence. However, worryingly, following prolonged adaptation to growth on human red blood cells, parasites were able to overcome their dependency on the sugar pathway and find another way into the human cell.
The researchers call for continued monitoring of the parasite to ensure that it has not switched to using a sugar-independent pathway to invade red blood cells--a likely prerequisite for human-to-human transmission.
Manoj Duraisingh, John LaPorte Given Professor of Immunology and Infectious Diseases, was the study's senior author. Other Harvard Chan School authors include Caeul Lim, Amy Bei, Rays Jiang, Saurabh Patel, Jonathan Goldberg, Yovany Moreno, and Maya Kono.
The study was supported by a National Institutes of Health grant AI091787 to M.T.D., a Center for Disease Control grant (R36-CK000119-01) and an Epidemiology of Infectious Disease and Biodefense Training Grant (2-T32-AI007535-12) to A.K.B., a National Institute of General Medical Sciences (NIGMS) Biotechnology Training Grant 5-T32-GM08334 to J.R.A. and National Institutes of Health Director's New Innovator Award 1DP2OD007124, NIGMS Center for Integrative Synthetic Biology Grant P50-GM098792, Ferry Award and Massachusetts Institute of Technology Startup funds to J.C.N. S.D.P. was supported by a National Institutes of Health Pediatric Scientist Development Program grant 5-K12-HD000850. Y.M. is a recipient of the Louis-Berlinguet Program Postdoctoral Research Scholarship in Genomics-Fonds de recherche du Quebec, Nature et technologies.
Reference: "Ancient human sialic acid variant restricts an emerging zoonotic malaria parasite," Selasi Dankwa, Caeul Lim, Amy K. Bei, Rays H.Y. Jiang, James R. Abshire, Saurabh D. Patel, Jonathan M. Goldberg, Yovany Moreno, Maya Kono, Jacquin C. Niles, and Manoj T. Duraisingh, Nature Communications, online April 4, 2016, doi: 10.1038/ncomms11187
Source: Harvard T.H. Chan School of Public Health
Beyond the Surface: Rethinking Environmental Hygiene Validation at Exchange25
June 30th 2025Environmental hygiene is about more than just shiny surfaces. At Exchange25, infection prevention experts urged the field to look deeper, rethink blame, and validate cleaning efforts across the entire care environment, not just EVS tasks.
A Controversial Reboot: New Vaccine Panel Faces Scrutiny, Support, and Sharp Divides
June 26th 2025As the newly appointed Advisory Committee on Immunization Practices (ACIP) met for the first time under sweeping changes by HHS Secretary Robert F. Kennedy Jr, the national spotlight turned to the panel’s legitimacy, vaccine guidance, and whether science or ideology would steer public health policy in a polarized era.
Getting Down and Dirty With PPE: Presentations at HSPA by Jill Holdsworth and Katie Belski
June 26th 2025In the heart of the hospital, decontamination technicians tackle one of health care’s dirtiest—and most vital—jobs. At HSPA 2025, 6 packed workshops led by experts Jill Holdsworth and Katie Belski spotlighted the crucial, often-overlooked art of PPE removal. The message was clear: proper doffing saves lives, starting with your own.