A research project coordinated by the UAB has succeeded in testing a vaccine against leishmaniasis. The vaccine was tested with the best animal model existing, the golden hamster, and can be produced at low costs by using insect larvae. The research, published in the latest edition of PLoS ONE, is an important step towards the fight against a disease which causes the death of 70,000 people each year in developing countries and of countless dogs, which also suffer from this disease and are its natural reservoir.
Leishmaniasis is one of the main health problems existing at global scale. It is produced by the protozoan Leishmania infantum, and canines, which can also be infected with the disease, are the main reservoirs of the organism. The disease is transmitted by insects from the Phlebotominae subfamily (sandflies), which are very similar to mosquitos. Clinical manifestations can go anywhere from minor skin sores to organ complications and, in the worst cases, death. The disease causes 70,000 deaths a year, mainly in developing countries, and affects over 12 million people around the world. Some 1.5 to 2 million new cases appear each year and an additional 350 million people are at risk of contracting the disease. Leishmaniasis is considered an endemic disease in parts of Asia, Africa, southern Europe, Mexico, Central America and South America. With the AIDS epidemic, leishmaniasis in humans has reactivated in many countries. Current treatments are unsatisfactory and, even though the most reasonable via to fight the disease would be to obtain an efficient vaccine, conventional vaccines until now have failed.
The research team led by the UAB compared the results of applying different vaccine strategies to the best animal model in which to study the human variant of the disease: the golden hamster. The results show that the most effective strategy is a combination of two vaccines created by the researchers: three doses of DNA with genes from the L. infantum and two doses of proteins coded by these genes.
The protein vaccine can be obtained at a low cost thanks to the use of insect larvae. To create the vaccine scientists isolated protozoan genes, inserted them into a virus which affects insects (baculovirus) and used these to infect larvae of a small worm, the cabbage looper (Trichoplusia ni). The larvae act as bioreactors and produce in large amounts and at a much lower cost than conventional reactors based on microorganisms the proteins which code these genes, and which are responsible for a protective response in vaccinated individuals. Using this method, a DNA vaccine can be manufactured with the protozoan protein coding genes, and a second vaccine with proteins associated with these genes (produced at a low cost using insect larvae), with the aim of increasing its effectiveness. This vaccine strategy can be used both in a preventive and a therapeutic manner, in both humans and dogs. Presently, the possibility of a translational phase is being contemplated with the aim of transferring as quickly as possible the results of this basic research to clinical practices, and of increasing the efficacy even more by using drugs to boost the immune response.
The research, directed by researcher Alhelà RodrÃguez and lecturer Jordi Alberola, both from the Department of Pharmacology, Therapeutics and Toxicology at the UAB, and members of the UAB Drug Analysis Service, included the participation of INIA (National Institute for Agrarian Technology and Research), the firm Alternative Gene Expression S.L., CReSA (Animal Health Research Centre, with the participation of the UAB and IRTA), and the University of Sao Paulo, Brazil. The research was published in the latest edition of the PLoS ONE journal.
Reference: Todolà F, et al. (2012) Head-to-Head Comparison of Three Vaccination Strategies Based on DNA and Raw Insect-Derived Recombinant Proteins against Leishmania. PLoS ONE 7(12): e51181. doi:10.1371/journal.pone.0009617
Â
Stay prepared and protected with Infection Control Today's newsletter, delivering essential updates, best practices, and expert insights for infection preventionists.
Reducing Hidden Risks: Why Sharps Injuries Still Go Unreported
July 18th 2025Despite being a well-known occupational hazard, sharps injuries continue to occur in health care facilities and are often underreported, underestimated, and inadequately addressed. A recent interview with sharps safety advocate Amanda Heitman, BSN, RN, CNOR, a perioperative educational consultant, reveals why change is overdue and what new tools and guidance can help.
New Study Explores Oral Vancomycin to Prevent C difficile Recurrence, But Questions Remain
July 17th 2025A new clinical trial explores the use of low-dose oral vancomycin to prevent Clostridioides difficile recurrence in high-risk patients taking antibiotics. While the data suggest a possible benefit, the findings stop short of statistical significance and raise red flags about vancomycin-resistant Enterococcus (VRE), underscoring the delicate balance between prevention and antimicrobial stewardship.
What Lies Beneath: Why Borescopes Are Essential for Verifying Surgical Instrument Cleanliness
July 16th 2025Despite their smooth, polished exteriors, surgical instruments often harbor dangerous contaminants deep inside their lumens. At the HSPA25 and APIC25 conferences, Cori L. Ofstead, MSPH, and her colleagues revealed why borescopes are an indispensable tool for sterile processing teams, offering the only reliable way to verify internal cleanliness and improve sterile processing effectiveness to prevent patient harm.
The Next Frontier in Infection Control: AI-Driven Operating Rooms
Published: July 15th 2025 | Updated: July 15th 2025Discover how AI-powered sensors, smart surveillance, and advanced analytics are revolutionizing infection prevention in the OR. Herman DeBoard, PhD, discusses how these technologies safeguard sterile fields, reduce SSIs, and help hospitals balance operational efficiency with patient safety.
Targeting Uncertainty: Why Pregnancy May Be the Best Time to Build Vaccine Confidence
July 15th 2025New national survey data reveal high uncertainty among pregnant individuals—especially first-time parents—about vaccinating their future children, underscoring the value of proactive engagement to strengthen infection prevention.