A new study by the Translational Genomics Research Institute (TGen) and Northern Arizona University (NAU) used deep DNA sequencing methods to generate the anthrax genome sequence from the victims of the 1979 anthrax outbreak in Sverdlovsk, Russia, when it was part of the USSR.
The Soviet Union produced anthrax spores on an industrial scale but repeatedly denied the existence of their biological weapons program. This study, to be published in the September issue of the journal mBio, represents a precise and detailed examination of the anthrax strain used in their weapons development, and includes an anthrax genetic database that puts the weapons strain into a global context.
"I have been studying this anthrax outbreak and these specimens for more than 20 years. Finally, using genomic technology, we could comprehensively characterize this pathogen genome," said Dr. Paul Keim, director of TGen's Pathogen Genomics Division, a Regents Professor of Biology and the Cowden Endowed Chair of Microbiology at NAU, and the study's lead author.
"This is the signature agent of the world's largest biological weapons program and now we have it in our genetic databases. Anywhere this strain shows up again, we will be able to identify it and track it back to its source. This is now an essential part of our forensic arsenal," said Keim, who also is director of NAU's Microbial Genetics & Genomics Center (MGGen).
The anthrax bacterium produces small capsules, or spores, that can lie dormant for decades. After settling inside the human lung, for example, it can cause a severe disease that, if not treated with antibiotics, kills 90 percent of those it infects.
Anthrax is found in many parts of the globe and dispersed through the human movement of animal parts contaminated with spores. Wool and hair from goats and sheep are moved globally as textiles or their precursors. When these originate in anthrax endemic regions, they can carry the spores, which are long-lived. While this bacterium has little variation from strain to strain, whole genome sequencing has identified DNA fingerprints that enable molecular epidemiology, tracing it to its source. When anthrax outbreaks occur, their whole genome profile are now routinely compared to the genetic database to identify possible sources and exclude others. This type of analysis was used by the FBI to track the spores in the 2001 anthrax letter attacks, which infected 22 people and killed five.
The Soviet Union had signed the Biological Weapons convention that prohibited the use of biological agents, including anthrax, as weapons. The United States' biological weapons program was eliminated in a decree by President Richard Nixon in 1969, but the Soviet program was maintained and expanded in a covert fashion for decades.
In 1992, an investigative team from the United States led by noted Harvard biologist Dr. Matt Meselson characterized the 1979 Sverdlovsk outbreak by interviewing local physicians, visiting cemeteries and examining autopsy specimens. This investigation, along with accounts by Ken Alibek, a former Soviet scientist, revealed that the Sverdlovsk anthrax outbreak was due to an industrial accident. A faulty filter at a Soviet spore production facility allowed anthrax spores, in a silent plume, to drift with the wind over the city and into the nearby countryside. Nearly 70 Sverdlovsk inhabitants died as far as three miles downwind from the facility, but more anthrax-susceptible farm animals died over 25 miles away. It remains the world's deadliest human outbreak of inhalation anthrax.
The bacterial genomes were generated from autopsy tissue specimens of two Sverdlovsk anthrax victims. These tissues were moved to the United States with permission of Sverdlovsk pathologists to continue the investigation into the disease outbreak. From these, it was established that the anthrax pathogen was detected within their tissues and the victims died from inhaling the spores.
The Sverdlovsk anthrax genome was compared to the global genome database maintained by NAU to identify its close relatives and to look for evidence of genetic engineering. The Flagstaff research team found that this strain was closely related to other Asian isolates with very few differences to naturally occurring anthrax. There were no signs of genetic engineering.
Keim notes that the Soviets had to be very meticulous to avoid mutant variants from dominating their production stock. Invariably when wild anthrax strains are grown extensively in the laboratory, they adapt to those conditions and lose the killing power.
"The Sverdlovsk strain's genome looked very much like those of wild strains we see across Asia," Keim said.
Meselson, who was not part of the current paper, notes: "If this strain had been grown repeated in the laboratory, it would have mutated to a form that had less virulence and less capacity to cause anthrax. The Soviet scientists must been very meticulous in their maintenance of the natural form."
Meselson, who is the Thomas Dudley Cabot Professor of the Natural Sciences at Harvard, is known for his 1961 discovery of messenger RNA.
This study was supported by a grant from the U.S. Department of Homeland Security.
Source: Translational Genomics Research Institute
Undermining Public Health: Nearly 1300 Cut From CDC With More to Come
February 14th 2025The Trump administration’s decision to terminate nearly 1,300 CDC employees weakens America’s public health defenses, jeopardizes critical research, and endangers the nation’s preparedness for future health crises.
Infection Intel: GermZapp Is the Innovative Hand Hygiene System IPC Needs
February 14th 2025GermZAPP is an affordable hand hygiene system designed to educate, monitor, and encourage adherence in schools, nursing homes, and health care settings, effectively addressing gaps in infection control.
Strengthening Infection Prevention: APIC Urges Support From HHS Secretary Kennedy
February 13th 2025APIC congratulates Secretary Robert F. Kennedy, Jr on his appointment and urges support for infection prevention priorities, including NHSN funding, workforce development, nursing home IPC improvements, medical device cleaning standards, and global health collaboration.
Universities and States Sue NIH Over Funding Cuts, Federal Judge Temporarily Halts Policy
February 13th 2025Twelve universities and 3 education groups filed a lawsuit against the NIH and HHS, challenging a 15% cap on research grant funding. A federal judge issued a temporary restraining order, halting the cuts while litigation proceeds. A hearing is set for February 21, 2025, and states and institutions are pushing for permanent relief.