Bacteriophages (phages) are probably the most abundant entities in nature, often exceeding bacterial densities by an order of magnitude. As viral predators of bacteria, phages have a major impact on bacterial communities by reducing some bacteria and enabling others to flourish. Phages also occasionally package host DNA and deliver it to other bacteria, in a process known as horizontal gene transfer (HGT).
The biology of phage infection has been extensively studied since the beginning of the 20th century. However, the fate of phages in complex bacterial communities resembling their natural ecosystem has not been studied at the cellular level. To investigate the biology of phage infection in complex bacterial communities, researchers followed phage dynamics in communities harboring phage-resistant (R) and phage-sensitive (S) bacteria, a common scenario in nature.
Now, in new research in the Jan. 12 edition of Cell, researchers at the Hebrew University of Jerusalem's Faculty of Medicine provide the first demonstration of a mechanism by which bacteria entirely resistant to a given phage become susceptible to it upon co-incubation with sensitive bacteria.
The researchers show how phage-sensitive bacteria harboring phage receptor can deliver the receptor to nearby phage-resistant cells that lack the phage receptor, via a molecular transfer they call "acquisition of sensitivity" (ASEN). This process involves a molecular exchange driven by membrane vesicles (MVs), in which phage-resistant cells transiently gain phage attachment molecules released from neighboring phage-sensitive cells. By exploiting this novel delivery system, phages can invade bacteria lacking their receptor.
The researchers further posit that this mechanism enables phages to expand their host range and deliver DNA into new species, thus facilitating the attachment of phages to non-host species, providing an as-yet unexplored route for horizontal gene transfer (HGT).
"In the present study, we show for the first time how bacteria entirely resistant to a given phage become susceptible upon co-incubation with sensitive bacteria. Phage invasion into resistant cells could have a major impact on transfer of antibiotic resistance and virulence genes among bacteria. This aspect should be carefully considered when employing phage therapy, as phage infection of a given species may result in gene transmission into neighboring bacteria resistant to the phage," said professor Sigal Ben-Yehuda, who led the research at the Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, in the Hebrew University's Faculty of Medicine.
"Our work indicates that, similarly to the remarkable arsenal of entry and spreading strategies employed by viruses, phages utilize alternative, as yet unidentified spreading mechanisms, which could expedite the infection process and promote phage spread within cells of the same and different species," said PhD student Elhanan Tzipilevich, who carried out this research.
Source: Hebrew University of Jerusalem
Dear Helpdesk: Working in a Toxic Health Care Environment
March 28th 2024Dear Helpdesk is your steadfast companion, offering life coaching and workplace advice from 2 seasoned IPs for some of your most challenging real-life situations. Let us help you navigate the intersection between work and life, guiding you to navigate the dynamic world of infection prevention with confidence and grace. This article is on handling a toxic health care environment.
Product Locator: Spring and Early Mother's Day Gift Guide for Infection Prevention Personnel
March 27th 2024Whether it's a spring holiday, birthdays, or no reason at all, infection prevention personnel love to give and receive gifts that help at the end of a stressful day. Infection Control Today® offers some gift ideas for infection prevention personnel and their families.
Catching Up With Vangie Dennis, AORN 2022-2023 President at AORN 2024
March 26th 2024Infection Control Today (ICT) had the privilege of catching up with Vangie Dennis, MSN, RN, CNOR, CMLSO, at the Association of periOperative Registered Nurses' (AORN’s) International Surgical Conference & Expo 2024. As the former president of AORN and an esteemed figure in perioperative services, Vangie Dennis shared insights into her recent endeavors and the exciting new chapter she's embarked upon.
How To Optimize Your Time Management Strategies for the Busy Infection Preventionist
March 25th 2024Is your calendar resembling a chaotic masterpiece of overlapping tasks? Join the club of infection preventionists striving to balance responsibilities. Dive into proven strategies from a fellow infection preventionist to reclaim control of your time, streamline tasks, and boost productivity effectively. This is an IP Lifeline article.